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Cytokeratin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16091

Novus Biologicals, part of Bio-Techne
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NBP2-16091

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 2 Antibody

Western Blot: Cytokeratin 2 Antibody [NBP2-16091]

Western Blot: Cytokeratin 2 Antibody [NBP2-16091]

Western Blot: Cytokeratin 2 Antibody [NBP2-16091] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP2-16091]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP2-16091]

Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP2-16091] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.
Cytokeratin 2 Antibody

Western Blot: Cytokeratin 2 Antibody [NBP2-16091] -

Western Blot: Cytokeratin 2 Antibody [NBP2-16091] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 2 antibody (NBP2-16091) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cytokeratin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 2

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Alternate Names

CK-2e, Cytokeratin-2e, epidermal ichthyosis bullosa of Siemens, Epithelial keratin-2e, K2e, keratin 2, keratin 2A (epidermal ichthyosis bullosa of Siemens), keratin, type II cytoskeletal 2 epidermal, Keratin-2 epidermis, Keratin-2e, KRT2AMGC116967, KRT2EMGC116968, KRTE, Type-II keratin Kb2

Gene Symbol

KRT2

UniProt

Additional Cytokeratin 2 Products

Product Documents for Cytokeratin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.