Cytokeratin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35131
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Cytokeratin 2 (KRT2) (NP_000414.2).
Sequence:
MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTKSISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQMNVGT
Sequence:
MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTKSISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQMNVGT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 2 Antibody
Western Blot: Cytokeratin 2 Antibody [NBP3-35131] -
Western Blot: Cytokeratin 2 Antibody [NBP3-35131] - Western blot analysis of various lysates using Cytokeratin 2(KRT2) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for Cytokeratin 2 Antibody
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 2
Alternate Names
CK-2e, Cytokeratin-2e, epidermal ichthyosis bullosa of Siemens, Epithelial keratin-2e, K2e, keratin 2, keratin 2A (epidermal ichthyosis bullosa of Siemens), keratin, type II cytoskeletal 2 epidermal, Keratin-2 epidermis, Keratin-2e, KRT2AMGC116967, KRT2EMGC116968, KRTE, Type-II keratin Kb2
Gene Symbol
KRT2
Additional Cytokeratin 2 Products
Product Documents for Cytokeratin 2 Antibody
Product Specific Notices for Cytokeratin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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