Cytokeratin 7 Antibody (5V4M10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16388

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16388-100ul
NBP3-16388-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5V4M10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 7 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 291-463 of human Cytokeratin 7 (KRT7) (P08729). QAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLAGDGVGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIRTASAS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 7 Antibody (5V4M10)

Western Blot: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Western Blot: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Western Blot: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388] - Western blot analysis of extracts of various cell lines, using Cytokeratin 7 (KRT7) Rabbit mAb (NBP3-16388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388] - Immunohistochemistry of paraffin-embedded mouse kidney using Cytokeratin 7 (KRT7) Rabbit mAb (NBP3-16388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (5V4M10) [NBP3-16388] - Immunohistochemistry of paraffin-embedded rat kidney using Cytokeratin 7 (KRT7) Rabbit mAb (NBP3-16388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Cytokeratin 7 Antibody (5V4M10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (5V4M10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 7 Antibody (5V4M10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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