Cytokeratin 7 Antibody (CL8725)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88919
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL8725
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINRRTAAENEFVVLKKDVD
Epitope
TAAENEFVVL
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Cytokeratin 7 Antibody (CL8725)
Western Blot: Cytokeratin 7 Antibody (CL8725) [NBP2-88919]
Western Blot: Cytokeratin 7 Antibody (CL8725) [NBP2-88919] - Analysis in human cell line RT-4.Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (CL8725) [NBP2-88919]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (CL8725) [NBP2-88919] - Analysis in human placenta and skeletal muscle tissues using NBP2-88919 antibody. Corresponding KRT7 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (CL8725) [NBP2-88919]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (CL8725) [NBP2-88919] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.Applications for Cytokeratin 7 Antibody (CL8725)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 7
Alternate Names
CK-7, CK7, K2C7, K7
Gene Symbol
KRT7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (CL8725)
Product Specific Notices for Cytokeratin 7 Antibody (CL8725)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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