Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49881
Recombinant Monoclonal Antibody.
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-1499R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-49881-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # KRT7/1499R
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)
Localization
Cytoplasmic
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Marker
Glandular and Transitional Epithelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-49880).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881] - Formalin-fixed, paraffin-embedded human Oviduct stained with Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).![Flow Cytometry: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-1499R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-49881-img0003.jpg "Flow Cytometry: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]")
Flow Cytometry: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881]
Flow Cytometry: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881] - Analysis of PFA-fixed HeLa cells using Cytokeratin 7 Rabbit Recombinant MAb (KRT7/1499R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red)![Western Blot: Cytokeratin 7 Antibody (KRT7/1499R)Azide and BSA Free [NBP2-49881]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-1499R-Azide-and-BSA-Free-Western-Blot-NBP2-49881-img0002.jpg "Western Blot: Cytokeratin 7 Antibody (KRT7/1499R)Azide and BSA Free [NBP2-49881]")
Western Blot: Cytokeratin 7 Antibody (KRT7/1499R)Azide and BSA Free [NBP2-49881]
Western Blot: Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free [NBP2-49881] - Analysis of human HeLa cell lysate using Cytokeratin 7 Rabbit Monoclonal Antibody (KRT7/1499R).Applications for Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Western Blot
1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 7
Alternate Names
CK-7, CK7, K2C7, K7
Gene Symbol
KRT7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free
Product Specific Notices for Cytokeratin 7 Antibody (KRT7/1499R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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