Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44819
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-760-+-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44819-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]")
![Western Blot: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-760-+-OV-TL12-30-Western-Blot-NBP2-44819-img0004.jpg "Western Blot: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # KRT7/760 + OV-TL12/30
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human KRT7 protein (KRT7/760); OTN 11, ovarian carcinoma cell line (OV-TL12/30) (Uniprot: P08729)
Localization
Cytoplasmic
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Marker
Glandular and Transitional Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG1 Kappa
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47943)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)
Western Blot: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]
Western Blot: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)![Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-760-+-OV-TL12-30-Immunocytochemistry-Immunofluorescence-NBP2-44819-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling KRT7 with Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-760-+-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44819-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]")
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30) [NBP2-44819] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760 + OV-TL12/30)Applications for Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)
Product Specific Notices for Cytokeratin 7 Antibody (KRT7/760 + OV-TL12/30)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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