Cytokeratin 7 Antibody (KRT7/903)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44817
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-903-Immunocytochemistry-Immunofluorescence-NBP2-44817-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Ferret (Negative), Mouse (Negative), Rabbit (Negative), Rat (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # KRT7/903
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)
Reactivity Notes
Does not react with Mouse, Rat, Ferret or Rabbit.
Localization
Cytoplasmic
Specificity
Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Marker
Glandular and Transitional Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11426)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 7 Antibody (KRT7/903)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817] - Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-903-Immunohistochemistry-Paraffin-NBP2-44817-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]")
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/903)![Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]](https://resources.bio-techne.com/images/products/Cytokeratin-7-Antibody-KRT7-903-Flow-Cytometry-NBP2-44817-img0003.jpg "Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]")
Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817]
Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) [NBP2-44817] - Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for Cytokeratin 7 Antibody (KRT7/903)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (KRT7/903)
Product Specific Notices for Cytokeratin 7 Antibody (KRT7/903)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...