Cytokeratin 7 Antibody (OV-TL12/30)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44814

Novus Biologicals, part of Bio-Techne
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NBP2-44814-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OV-TL12/30

Concentration

0.2 mg/ml

View all Cytokeratin 7 Antibodies »

Product Specifications

Immunogen

OTN 11, ovarian carcinoma cell line

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Localization

Cytoplasmic

Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Marker

Glandular and Transitional Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47940)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 7 Antibody (OV-TL12/30)

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
View 7 more images

Applications for Cytokeratin 7 Antibody (OV-TL12/30)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

1.0 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

UniProt

P08729 (Human)

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (OV-TL12/30)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 7 Antibody (OV-TL12/30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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