Cytokeratin 7 Antibody (RCK105) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-9021

Novus Biologicals, part of Bio-Techne
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NB120-9021
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine, Feline, Ferret, Hamster

Cited:

Ferret

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry Whole-Mount

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RCK105

Format

BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 7 Antibodies »

Product Specifications

Immunogen

Cytokeratins from the human bladder carcinoma cell line T24.

Reactivity Notes

Ferret reactivity reported in scientific literature (PMID: 28977592).

Localization

Cytoplasmic

Specificity

Reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Cytokeratin 7 Antibody (RCK105) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:200

Western Blot

1:100-1:1000
Application Notes
Use in IHC-Whole mount reported in scientific literature (PMID: 28977592).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 7

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Alternate Names

CK-7, CK7, K2C7, K7

Entrez Gene IDs

3855 (Human); 110310 (Mouse); 300242 (Rat); 477602 (Canine)

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (RCK105) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 7 Antibody (RCK105) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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