Cytokeratin 7 Antibody (rOV-TL12/30)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53150

Novus Biologicals, part of Bio-Techne
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NBP2-53150-100ug
NBP2-53150-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # rOV-TL12/30

Concentration

0.2 mg/ml

View all Cytokeratin 7 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)

Localization

Cytoplasmic

Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Marker

Glandular and Transitional Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of recombinant monoclonal antibody purified by Protein A or G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. (NBP2-54499)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 7 Antibody (rOV-TL12/30)

Western Blot: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Western Blot: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Western Blot: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (rOV-TL12/30).
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cytokeratin-7 Recombinant Mouse Monoclonal Antibody (rOV-TL12/30).
Flow Cytometry: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Flow Cytometry: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150]

Flow Cytometry: Cytokeratin 7 Antibody (rOV-TL12/30) [NBP2-53150] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (rOV-TL12/30).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
View 1 more images

Applications for Cytokeratin 7 Antibody (rOV-TL12/30)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

UniProt

P08729 (Human)

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (rOV-TL12/30)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 7 Antibody (rOV-TL12/30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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