Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48179
![Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted [NBP2-48179]](https://resources.bio-techne.com/images/products/Cytokeratin-8-18-Antibody-C-51-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48179-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted [NBP2-48179]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Chicken (Negative), Donkey (Negative), Ferret (Negative), Monkey, Mouse (Negative), Rabbit (Negative), Sheep, Xenopus (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-51
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cytoskeleton preparation from HeLa cells
Reactivity Notes
Does not react with Mouse, Ferret, Rabbit, donkey, Chicken or Xenopus laevis.
Rat reactivity reported in scientific liteRature (PMID: 28975767).
Rat reactivity reported in scientific liteRature (PMID: 28975767).
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted [NBP2-48179]
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted [NBP2-48179] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (C-51).Applications for Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 17q21.2 (CK8); 12q13.13 (CK18) Molecular weight of antigen: 52.5kDa (CK8); 45kDa (CK18) Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 8/18
Alternate Names
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbol
KRT8
Additional Cytokeratin 8/18 Products
Product Documents for Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted
Product Specific Notices for Cytokeratin 8/18 Antibody (C-51) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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