Cytokeratin 8 Antibody (AE3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-9287
![Flow Cytometry: Cytokeratin 8 Antibody (AE3) - BSA Free [NB120-9287]](https://resources.bio-techne.com/images/products/Cytokeratin-8-Antibody-AE3-Flow-Cytometry-NB120-9287-img0001.jpg "Flow Cytometry: Cytokeratin 8 Antibody (AE3) - BSA Free [NB120-9287]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Chicken, Primate, Rabbit
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AE3
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human epidermal cells
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Cell Membrane and Cytoplasmic
Specificity
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development.
Marker
Epithelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Cytokeratin 8 Antibody (AE3) - BSA Free
Flow Cytometry: Cytokeratin 8 Antibody (AE3) - BSA Free [NB120-9287]
Flow Cytometry: Cytokeratin 8 Antibody (AE3) [NB120-9287] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Applications for Cytokeratin 8 Antibody (AE3) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This antibody is suitable for staining formalin fixed paraffin wax embedded sections following enzyme digestion at a recommended dilution of at least 1:100. This antibody reacts with MCF-7, RT 112 & A 431 cultured cell lines. Use in FLOW reported in scientific literature (PMID 28497488 and 25468832).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 8
Alternate Names
CARD2, CK8, EndoA, KRT8
Gene Symbol
KRT8
Additional Cytokeratin 8 Products
Product Documents for Cytokeratin 8 Antibody (AE3) - BSA Free
Product Specific Notices for Cytokeratin 8 Antibody (AE3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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