Cytokeratin 8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16094

Novus Biologicals, part of Bio-Techne
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NBP2-16094
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 8 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Porcine (83%), Bovine (84%), Canine (89%).

Localization

Cytoplasm

Marker

Epithelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 8 Antibody

Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094]

Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094]

Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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Applications for Cytokeratin 8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Application Notes
IHC-WM-Assay dependent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-16094 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

UniProt

P05787 (Human)

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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