Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34533

Novus Biologicals, part of Bio-Techne
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NBP2-34533-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 34BE12

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin, HMW Antibodies »

Product Specifications

Immunogen

Solubilized keratin extract from human stratum corneum

Localization

Cytoplasmic

Specificity

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34340).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

Western Blot: Cytokeratin, HMW Antibody (34BE12)Azide and BSA Free [NBP2-34533]

Western Blot: Cytokeratin, HMW Antibody (34BE12)Azide and BSA Free [NBP2-34533]

Western Blot: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Western Blot Analysis of MCF-7 cell lysate using Cytokeratin, HMW Antibody (34BE12).
Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533]

Immunocytochemistry/Immunofluorescence: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Cytokeratin with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin, HMW MAb (34bE12).
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Applications for Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1.0ug/ml

Immunohistochemistry-Frozen

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin, HMW

KRT76, also known as Keratin, type II cytoskeletal 2 oral, is a 638 amino acid that is approx. 66 kDa, belongs to the intermediate filament family responsible for the structural integrity of epithelial cells, subdivided into epithelial keratins and hair keratins, and probably contributes to terminal cornification. Studies are being performed in relation to this protein and obstructive jaundice, pharyngitis, jaundice, cervical cancer, neuronitis, colorectal cancer, cervicitis, interferon and hepatitis. This protein has been shown to have interactions with ATG13, PDPK1, ULK2, GABARAP, and PRKAA1 in cytoskeleton organization pathways.

Alternate Names

CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Gene Symbol

KRT76

Additional Cytokeratin, HMW Products

Product Documents for Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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