Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08361
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]](https://resources.bio-techne.com/images/products/Cytokeratin-HMW-Antibody-KRTH-2147R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08361-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # KRTH/2147R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Solubilized keratin extract from human stratum corneum
Localization
Cytoplasmic
Specificity
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07272).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]
Immunocytochemistry/Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361] - Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]](https://resources.bio-techne.com/images/products/Cytokeratin-HMW-Antibody-KRTH-2147R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08361-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]")
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361]
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free [NBP3-08361] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R).Applications for Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin, HMW
Alternate Names
CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9
Gene Symbol
KRT76
Additional Cytokeratin, HMW Products
Product Documents for Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free
Product Specific Notices for Cytokeratin, HMW Antibody (KRTH/2147R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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