Cytokeratin, LMW Antibody (rKRTL/6616)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07644

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rKRTL/6616

Concentration

0.2 mg/ml

View all Cytokeratin, LMW Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to Cytokeratin, LMW residues within aa100-200 of Cytokeratin, LMW (Uniprot: Q7Z794)

Localization

Cytoplasmic

Specificity

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08674)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cytokeratin, LMW Antibody (rKRTL/6616)

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644] - Formalin-fixed, paraffin-embedded human sebaceous gland stained with Cytokeratin, LMW Recombinant Mouse Monoclonal Antibody (rKRTL/6616).
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/6616) [NBP3-07644] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, LMW Recombinant Mouse Monoclonal Antibody (rKRTL/6616).

Applications for Cytokeratin, LMW Antibody (rKRTL/6616)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, LMW

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

UniProt

Q7Z794 (Human)

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (rKRTL/6616)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin, LMW Antibody (rKRTL/6616)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.