Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11443

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # KRTL/1077 + KRTH/1076

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Cytokeratin, pan Antibodies »

Product Specifications

Immunogen

Recombinant human KRT77 and KRT76 protein

Reactivity Notes

Shows broad species reactivity.

Localization

Cytoplasmic

Specificity

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44367).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] - Rat Stomach stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).
Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] - Human Breast Carcinoma stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).
Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] -

Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRTL/1077 + KRTH/1076) - Azide and BSA Free [NBP3-11443] - Rat Oviduct with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1 - 0.2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin, pan

Cytokeratins are a family of intermediate filamentous proteins that are expressed by epithelial cells (1,2). Cytokeratins range in size with a theoretic molecular weight varying from approximately 40 kDa to 68 kDa (2,3). The cytokeratin family consists of 20 polypeptides that are further divided into two main groups based on isoelectric point and molecular weight (1-3). The type I group are smaller, acidic polypeptides designated as cytokeratin 9 through cytokeratin 20 (CK9 - CK20) (1-4). Conversely, CK1 - CK8 belong to the type II group, classified as larger, basic or neutral polypeptides (1-4). Structurally, cytokeratins have homologous basic structure with other intermediate filaments; they possess a 300-315 amino acid (aa) central helical region that consists of four conserved domains (1A, 2A, 1B, and 2B) which are separated by linker domains (L1, L12, and L2) (1,5). Additionally, flanking this central region, both the amino- and carboxyl-terminal ends have a homologous subdomain (H), a variable domain (V), and charged end subdomains (E) (1). Furthermore, the central rod of one cytokeratin monomer binds with another monomer to form a coiled-coil dimer which subsequently binds another dimer to form a tertramer (3). Finally, many tetramers join together to ultimately form an intermediate filament of approximately 10nm in diameter (1-3, 5). Cytokeratins are expressed as pairs, typically with a type I and type II member; for example, CK10 pairs with CK1 (1,3).

Epithelial cells express multiple subtypes of cytokeratins which can be used to classify epithelial cell type or differentiation status, as well tumor progression or diagnosis (2). Cytokeratins are important for both stability and integrity of epithelial cells and function in intracellular signaling, from wound healing to apoptosis (1). Cytokeratins are useful immunohistochemistry tumor markers and antibodies to cytokeratins are a common pathological tool (1,3,6). Cytokeratin pan antibody is an antibody cocktail mixture that can detect multiple cytokeratins and reacts to multiple epithelial tissues (1,3,6). For example, AE-1/AE-3 is a commonly used specific pan cytokeratin that detects cytokeratins 1-8, 10, 14-16 and 19 (1,3,6).

Given the role of cytokeratins in the structural integrity of epithelial cells, mutations in cytokeratins have been shown to play a role in a variety of human diseases including epidermolysis bullosa simplex (EBS) (4,5). EBS is an autosomal dominant disorder that is caused by missense mutations in either CK5 or CK14 (5). Other known cytokeratin-related disorders include bullous ichthyosis, a skin disorder characterized by redness, blistering, and hyperkeratosis, and epidermolytic palmoplantar keratoderma (EPPK), which results in hyperkeratosis on the palms and soles of the body (7).

References

1. Awasthi, P., Thahriani, A., Bhattacharya, A., Awasthi, P., & Keratins, B. A. (2016). Keratins or cytokeratins: a review article. Journal of Advanced Medical and Dental Sciences Research. https://10.21276/jamdsr.2016.4.4.30

2. Southgate, J., Harnden, P., & Trejdosiewicz, L. K. (1999). Cytokeratin expression patterns in normal and malignant urothelium: a review of the biological and diagnostic implications. Histology and histopathology. https://doi.org/10.14670/HH-14.657

3. Belaldavar, C., Mane, D. R., Hallikerimath, S., Kale, A. D. (2016). Cytokeratins: Its role and expression profile in oral health and disease. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. https://doi.org/10.1016/j.ajoms.2015.08.001.

4. Linder S. (2007). Cytokeratin markers come of age. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1159/000107582

5. Jacob, J. T., Coulombe, P. A., Kwan, R., & Omary, M. B. (2018). Types I and II Keratin Intermediate Filaments. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a018275

6. Ordonez N. G. (2013). Broad-spectrum immunohistochemical epithelial markers: a review. Human pathology. https://doi.org/10.1016/j.humpath.2012.11.016

7. McLean, W. H., & Moore, C. B. (2011). Keratin disorders: from gene to therapy. Human molecular genetics. https://doi.org/10.1093/hmg/ddr379

Alternate Names

AEI2, CK1, EHK, EPPK, K1, KRT1A, NEPPK

Gene Symbol

KRT1

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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