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Cytomegalovirus pp65 Antibody (B051M) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-68199

Novus Biologicals, part of Bio-Techne
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NB110-68199

Key Product Details

Species Reactivity

Cytomegalovirus, Virus

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B051M

Format

BSA Free

Concentration

1.3 mg/ml

Product Specifications

Immunogen

CMV Lysate

Specificity

Specific for pp65 of CMV. Functions in pp65 antigenemia assay (indirect IFA). (B051M)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Cytomegalovirus pp65 Antibody (B051M) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.01 M Phosphate Buffered Saline, pH 7.2. Product contains no stabilizing proteins.

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Cytomegalovirus pp65

Human cytomegalovirus, also called HCMV or human herpesvirus 5, is a double-stranded DNA, enveloped virus of 230-250 kilobases (kb) that is a member of the Herpesviridae family and the subfamily Betaherpesvirinae (1,2). Other herpesviruses, which share the characteristic of lifelong latency following primary infection, include herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), varicella zoster virus (VSV), and Epstein-Barr virus (EBV) (1). Cytomegalovirus entry into cells is mediated by a trimeric complex of glycoproteins gH, gL, and gO and a pentamer made of proteins gH, gL, UL128, UL130, and UL131A (1,3). The trimer binds PDGFRalpha for entry into fibroblasts while the pentamer binds neuropilin-2 for entry into endothelial and epithelial cells (1,4). Additionally, the fusion protein glycoprotein B (gB) plays a pivotal role for cytomegalovirus entry into the host cell membrane (1,3).

Cytomegalovirus is a common virus, infecting ~60% of adults in developed countries and over 90% in developing countries, as indicated by presence of specific IgG antibodies (2-4). Although a majority of people infected remain asymptomatic, immunocompromised people, organ transplant recipients, and fetuses and newborns are more susceptible to HCMV-associated diseases (1-4). Annually, up to 2% of newborns are born with HCMV, making it the most common congenital infection (2-4). Cytomegalovirus is known to cause sensorineural hearing loss (SNHL), mental retardation, chorioretinitis, skin lesions, as well as end organ disease (3,5). There are a few CMV therapeutics approved by FDA including valganciclovir and letermovir; however, despite some benefits, there is the need for new antivirals and combination therapies (5). Potential new treatment options include monoclonal antibodies and sirtuins (5).

References

1. Connolly SA, Jardetzky TS, Longnecker R. The structural basis of herpesvirus entry. Nat Rev Microbiol. 2021;19(2):110-121. https://doi.org/10.1038/s41579-020-00448-w

2. Griffiths P, Reeves M. Pathogenesis of human cytomegalovirus in the immunocompromised host. Nat Rev Microbiol. 2021;19(12):759-773. https://doi.org/10.1038/s41579-021-00582-z

3. Krstanovic F, Britt WJ, Jonjic S, Brizic I. Cytomegalovirus Infection and Inflammation in Developing Brain. Viruses. 2021;13(6):1078. Published 2021 Jun 4. https://doi.org/10.3390/v13061078

4. Griffiths P, Baraniak I, Reeves M. The pathogenesis of human cytomegalovirus. J Pathol. 2015;235(2):288-297. https://doi.org/10.1002/path.4437

5. Acosta E, Bowlin T, Brooks J, et al. Advances in the Development of Therapeutics for Cytomegalovirus Infections. J Infect Dis. 2020;221(Suppl 1):S32-S44. https://doi.org/10.1093/infdis/jiz493

Alternate Names

64 kDa lower matrix phosphoprotein, 65 kDa lower matrix phosphoprotein, CMV, CMV 65 kDa lower matrix phosphoprotein, CMV pp65, CMV pp65 IE, CMV tegument protein UL83, cytomegalovirus, Cytomegalovirus 65 kDa lower matrix phosphoprotein, Cytomegalovirus pp65, Cytomegalovirus tegument protein UL83, GP64, PP64, PP65, Tegument protein UL 83, Tegument protein UL83, UL 83, UL83

Additional Cytomegalovirus pp65 Products

Product Documents for Cytomegalovirus pp65 Antibody (B051M) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytomegalovirus pp65 Antibody (B051M) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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