Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72100
![Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Western-Blot-NBP2-72100-img0001.jpg "Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)Azide and BSA Free [NBP2-72100]")
![Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Western-Blot-NBP2-72100-img0002.jpg "Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)Azide and BSA Free [NBP2-72100]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1F1
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 (NP_066024) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1)Azide and BSA Free [NBP2-72100]
Western Blot: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0011.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]
Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100] - Staining of paraffin-embedded Human liver tissue using anti-GBA3 mouse monoclonal antibody.![Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72100-img0005.jpg "Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]
Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GBA3 antibody, and then analyzed by flow cytometry.![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0006.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0007.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0008.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0009.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0010.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72100-img0012.jpg "Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72100-img0003.jpg "Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
![Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]](https://resources.bio-techne.com/images/products/Cytosolic-beta-Glucosidase-GBA3-Antibody-OTI1F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72100-img0004.jpg "Flow Cytometry: Cytosolic beta-Glucosidase/GBA3 Antibody (OTI1F1) - Azide and BSA Free [NBP2-72100]")
Applications
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytosolic beta-Glucosidase/GBA3
Alternate Names
CBGL1, Cytosolic betaGlucosidase, GBA3, GLUC, KLrP
Gene Symbol
GBA3
Additional Cytosolic beta-Glucosidase/GBA3 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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