D-Dimer Antibody (28) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05045
![ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]](https://resources.bio-techne.com/images/products/D-Dimer-Antibody-28-BSA-Free-ELISA-NBP1-05045-img0004.jpg "ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 28
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human D-dimer
Epitope
The epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.
Specificity
Binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly
coated fibrinogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for D-Dimer Antibody (28) - BSA Free
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]
ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.Applications for D-Dimer Antibody (28) - BSA Free
Application
Recommended Usage
ELISA
1:8000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05045 binds free D-dimer in solution. NBP1-05045 works as a capture antibody in sandwich ELISA with biotinylated NBP1-05045 or NBP2-21616 as a detection antibody. Biotinylated NBP1-05045 works as a detection antibody in sandwich ELISA with NBP2-21616 or NBP1-05045 as a capture antibody. The combination with NBP1-05045 as a capture antibody and NBP2-23592 as a detection antibody is recommended.
WB: Due to reducing conditions in Western blotting NBP1-05045 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex. Use in IHC-P reported in scientific literature (PMID:33480806)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: D-dimer
Long Name
D-Dimer breakdown product of Fibrinogen
Alternate Names
Ddimer, FDP
Additional D-dimer Products
Product Documents for D-Dimer Antibody (28) - BSA Free
Product Specific Notices for D-Dimer Antibody (28) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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