DAD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-669

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DAD1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a fourteen amino acid sequence within the C-Terminal region of human DAD1

Reactivity Notes

Predicted cross-reactivities: Mouse

Localization

Cytoplasmic (microsome)

Specificity

DAD1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAD1 Antibody

Western Blot: DAD1 AntibodyBSA Free [NB600-669]

Western Blot: DAD1 AntibodyBSA Free [NB600-669]

Western Blot: DAD1 Antibody [NB600-669] - Analysis of whole cell lysates from HepG2 human hepatocellular carcinoma cells probed with Rabbit anti Human DAD1
Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NB600-669]

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody - BSA Free [NB600-669]

Immunocytochemistry/Immunofluorescence: DAD1 Antibody [NB600-669] - Immunocytochemical stainning of HepG2 cells with Rabbit anti Human DAD1

Applications for DAD1 Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
WB: Use at a concentration of 0.5 - 2.0 ug/ml. Detects a band of approximately 22 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAD1

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Alternate Names

DAD-1, defender against cell death 1EC 2.4.1.119, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, oligosaccharyltransferase 2 homolog, OST2

Entrez Gene IDs

1603 (Human); 13135 (Mouse)

Gene Symbol

DAD1

UniProt

P61803 (Human)

Additional DAD1 Products

Product Documents for DAD1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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