DAD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82466

Novus Biologicals, part of Bio-Techne
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NBP1-82466
NBP1-82466-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DAD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSASVVSVISRFLEEYLSSTPQRLKLLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAD1 Antibody

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody [NBP1-82466]

Immunocytochemistry/ Immunofluorescence: DAD1 Antibody [NBP1-82466]

Immunocytochemistry/Immunofluorescence: DAD1 Antibody [NBP1-82466] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DAD1 Antibody [NBP1-82466]

Immunohistochemistry-Paraffin: DAD1 Antibody [NBP1-82466]

Immunohistochemistry-Paraffin: DAD1 Antibody [NBP1-82466] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for DAD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAD1

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Alternate Names

DAD-1, defender against cell death 1EC 2.4.1.119, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, oligosaccharyltransferase 2 homolog, OST2

Gene Symbol

DAD1

Additional DAD1 Products

Product Documents for DAD1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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