DAPK3/ZIPK Antibody (PSH02-18)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33113
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-18
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human DAPK3/ZIPK aa 250-454 (O43293). (Uniprot: O43293)
Localization
Nucleus, Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DAPK3/ZIPK Antibody (PSH02-18)
Western Blot: DAPK3/ZIPK Antibody (PSH02-18) [NBP3-33113]
Western Blot: DAPK3/ZIPK Antibody (PSH02-18) [NBP3-33113] - Western blot analysis of DAPK3/ZIPK on different lysates with Rabbit anti-DAPK3/ZIPK antibody (NBP3-33113) at 1/1,000 dilution.Lane 1: A431 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: SiHa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Hela cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Caco-2 cell lysate
Lane 9: K-562 cell lysate
Lane 10: MCF7 cell lysate
Lane 11: U-2 OS cell lysate
Lane 12: Human kidney tissue lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33113) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for DAPK3/ZIPK Antibody (PSH02-18)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DAPK3/ZIPK
Long Name
Death-associated Protein Kinase 3
Alternate Names
Dlk, ZIPK
Gene Symbol
DAPK3
Additional DAPK3/ZIPK Products
Product Documents for DAPK3/ZIPK Antibody (PSH02-18)
Product Specific Notices for DAPK3/ZIPK Antibody (PSH02-18)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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