DAPP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27542

Novus Biologicals, part of Bio-Techne
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NBP2-27542
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DAPP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLSQIRKQLNQGE corresponding to internal region (near C-Terminus) according to NP_055210.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DAPP1 Antibody

Western Blot: DAPP1 Antibody [NBP2-27542]

Western Blot: DAPP1 Antibody [NBP2-27542]

Western Blot: DAPP1 Antibody [NBP2-27542] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human DAPP1 with DYKDDDDK tag probed with NBP2-27542 (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with MBP2-27542 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for DAPP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human DAPP1 (myc and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAPP1

B cell adapter molecule (BAM32) is also designated a dual adapter for phosphotyrosine and 3-phosphotyrosine, and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC gamma2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.

Long Name

Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1

Alternate Names

BAM32

Gene Symbol

DAPP1

UniProt

NP_055210.2 (Human)

Additional DAPP1 Products

Product Documents for DAPP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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