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DARS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00268

Novus Biologicals, part of Bio-Techne
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NBP3-00268-100ul
NBP3-00268-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human DARS (Accession#: P14868; Met1-Pro501)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DARS Antibody

Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268]

Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268]

Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: DARS Antibody [NBP3-00268]

Immunoprecipitation: DARS Antibody [NBP3-00268]

Immunoprecipitation: DARS Antibody [NBP3-00268] - DARS was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 ul anti-DARS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DARS rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa

Applications for DARS Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DARS

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Alternate Names

aspartate tRNA ligase 1, cytoplasmic, Aspartate--tRNA ligase, aspartyl-tRNA synthetase, aspartyl-tRNA synthetase, cytoplasmic, AspRS, Cell proliferation-inducing gene 40 protein, cell proliferation-inducing protein 40, DKFZp781B11202, EC 6.1.1.12, MGC111579

Gene Symbol

DARS1

Additional DARS Products

Product Documents for DARS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DARS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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