DARS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83854

Novus Biologicals, part of Bio-Techne
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NBP1-83854
NBP1-83854-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DARS2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DARS2 Antibody

Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854]

Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854]

Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human colon, kidney, liver and lymph node using Anti-DARS2 antibody NBP1-83854 (A) shows similar protein distribution across tissues to independent antibody NBP1-83855 (B).
Western Blot: DARS2 Antibody [NBP1-83854]

Western Blot: DARS2 Antibody [NBP1-83854]

Western Blot: DARS2 Antibody [NBP1-83854] - Analysis using Anti-DARS2 antibody NBP1-83854 (A) shows similar pattern to independent antibody NBP1-83855 (B).
Immunocytochemistry/ Immunofluorescence: DARS2 Antibody [NBP1-83854]

Immunocytochemistry/ Immunofluorescence: DARS2 Antibody [NBP1-83854]

Immunocytochemistry/Immunofluorescence: DARS2 Antibody [NBP1-83854] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
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Applications for DARS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DARS2

The protein encoded by the DARS2 gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). (provided by RefSeq)

Alternate Names

aspartate tRNA ligase 2, mitochondrial, Aspartate--tRNA ligase, aspartyl-tRNA synthetase 2, mitochondrial, aspartyl-tRNA synthetase, mitochondrial, ASPRS, ASPRS. LBSL, EC 6.1.1, EC 6.1.1.12, FLJ10514, LBSL, MT-ASPRS, RP3-383J4.2

Gene Symbol

DARS2

Additional DARS2 Products

Product Documents for DARS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DARS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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