Daxx Antibody (PCRP-DAXX-5G11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13861

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-DAXX-5G11

Concentration

0.2 mg/ml

View all Daxx Antibodies »

Product Summary for Daxx Antibody (PCRP-DAXX-5G11)

Immunogen

Recombinant full-length human Daxx protein (Uniprot: Q9UER7)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

81.37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14025)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Daxx Antibody (PCRP-DAXX-5G11)

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Immunocytochemistry/Immunofluorescence: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861] - Immunofluorescence analysis of PFA-fixed HeLa cells. Daxx Antibody (PCRP-DAXX-5G11) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861] - Formalin-fixed, paraffin-embedded human thyroid stained with Daxx antibody (PCRP-DAXX-5G11) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Flow Cytometry: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Flow Cytometry: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861]

Flow Cytometry: Daxx Antibody (PCRP-DAXX-5G11) [NBP3-13861] - Flow cytometric analysis of PFA-fixed HeLa cells. Daxx antibody (PCRP-DAXX-5G11) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Applications for Daxx Antibody (PCRP-DAXX-5G11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Daxx

Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi). The promyelocytic leukemia protein Pml is an essential component of the PML-NB. Data suggests that certain stimuli including Fas stimulation, causes Daxx to be translocated from the nucleus to the cytoplasm. Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes. At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus. Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells. Pml is necessary for Fas-induced cell death and Daxx pro-apoptotic functions. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Daxx pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated, research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders. Human Daxx is a 740 amino acid protein (GenBank no. gi/62898369).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Gene Symbol

DAXX

UniProt

Q9UER7

Additional Daxx Products

Product Documents for Daxx Antibody (PCRP-DAXX-5G11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Daxx Antibody (PCRP-DAXX-5G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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