DAZAP1 Antibody (2F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00026528-M03

Novus Biologicals, part of Bio-Techne
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H00026528-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DAZAP1 Antibodies »

Product Specifications

Immunogen

DAZAP1 (NP_061832.2, 308 a.a. ~ 407 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVPPPPATPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR

Specificity

Reacts with DAZ associated protein 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DAZAP1 Antibody (2F6)

Western Blot: DAZAP1 Antibody (2F6) [H00026528-M03]

Western Blot: DAZAP1 Antibody (2F6) [H00026528-M03]

Western Blot: DAZAP1 Antibody (2F6) [H00026528-M03] - Analysis of DAZAP1 expression in transfected 293T cell line by DAZAP1 monoclonal antibody (M03), clone 2F6. Lane 1: DAZAP1 transfected lysate (Predicted MW: 43.4 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DAZAP1 Antibody (2F6) [H00026528-M03]

Immunocytochemistry/ Immunofluorescence: DAZAP1 Antibody (2F6) [H00026528-M03]

Immunocytochemistry/Immunofluorescence: DAZAP1 Antibody (2F6) [H00026528-M03] - Analysis of monoclonal antibody to DAZAP1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: DAZAP1 Antibody (2F6) [H00026528-M03] - Detection limit for recombinant GST tagged DAZAP1 is 0.03 ng/ml as a capture antibody.

Applications for DAZAP1 Antibody (2F6)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DAZAP1

In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Alternate Names

DAZ associated protein 1, DAZ-associated protein 1, deleted in azoospermia associated protein 1, Deleted in azoospermia-associated protein 1, MGC19907

Entrez Gene IDs

26528 (Human)

Gene Symbol

DAZAP1

UniProt

NP_061832 (Human)

Additional DAZAP1 Products

Product Documents for DAZAP1 Antibody (2F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAZAP1 Antibody (2F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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