DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor® 488]

Novus Biologicals, part of Bio-Techne | Catalog # DDX0207A488

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 109H12.03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DC-SIGN/CD209 Antibodies »

Product Specifications

Immunogen

human DC-SIGN-transfected HeLa cells

Reactivity Notes

Human

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor® 488]

Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor (R) 488] [DDX0207A488] - A) FACS staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.
Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor (R) 488] [DDX0207A488] - FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)

Applications for DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor® 488]

Application
Recommended Usage

Flow (Cell Surface)

1:10-1:1000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10-1:500
Application Notes
Immunohistochemistry, Flow Cytometry
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

Antibody in PBS and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: DC-SIGN/CD209

DC-SIGN encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Gene Symbol

CD209

Additional DC-SIGN/CD209 Products

Product Documents for DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor® 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DC-SIGN/CD209 Antibody (109H12.03) [Alexa Fluor® 488]

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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