DCK Antibody (OTI3F5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00590

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F5

Concentration

1 mg/ml

View all DCK Antibodies »

Product Summary for DCK Antibody (OTI3F5)

Immunogen

Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DCK Antibody (OTI3F5)

Western Blot: DCK Antibody (OTI3F5) [NBP2-00590]

Western Blot: DCK Antibody (OTI3F5) [NBP2-00590]

Western Blot: DCK Antibody (OTI3F5) [NBP2-00590] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunocytochemistry/ Immunofluorescence: DCK Antibody (OTI3F5) [NBP2-00590]

Immunocytochemistry/ Immunofluorescence: DCK Antibody (OTI3F5) [NBP2-00590]

Immunocytochemistry/Immunofluorescence: DCK Antibody (OTI3F5) [NBP2-00590] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK.
Immunohistochemistry-Paraffin: DCK Antibody (OTI3F5) [NBP2-00590]

Immunohistochemistry-Paraffin: DCK Antibody (OTI3F5) [NBP2-00590]

Immunohistochemistry-Paraffin: DCK Antibody (3F5) [NBP2-00590] - Staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody.
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Applications for DCK Antibody (OTI3F5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DCK

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq].

Long Name

Deoxycytidine kinase

Alternate Names

EC 2.7.1.113, EC 2.7.1.74, EC 2.7.1.76

Entrez Gene IDs

1633 (Human)

Gene Symbol

DCK

Additional DCK Products

Product Documents for DCK Antibody (OTI3F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DCK Antibody (OTI3F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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