DCUN1D2 Antibody (001)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90306
Recombinant Monoclonal Antibody
![Western Blot: DCUN1D2 Antibody (001) [NBP2-90306]](https://resources.bio-techne.com/images/products/DCUN1D2-Antibody-001-Western-Blot-NBP2-90306-img0003.jpg "Western Blot: DCUN1D2 Antibody (001) [NBP2-90306]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human DCUN1D2 (Accession#: NP_001014305.1; Met1-Phe259).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for DCUN1D2 Antibody (001)
Western Blot: DCUN1D2 Antibody (001) [NBP2-90306]
Western Blot: DCUN1D2 Antibody (001) [NBP2-90306] - Lane A: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa![Immunoprecipitation: DCUN1D2 Antibody (001) [NBP2-90306]](https://resources.bio-techne.com/images/products/DCUN1D2-Antibody-001-Immunoprecipitation-NBP2-90306-img0002.jpg "Immunoprecipitation: DCUN1D2 Antibody (001) [NBP2-90306]")
Immunoprecipitation: DCUN1D2 Antibody (001) [NBP2-90306]
Immunoprecipitation: DCUN1D2 Antibody (001) [NBP2-90306] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg HuT78 Whole Cell Lysate2 uL anti-DCUN1D2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDaApplications for DCUN1D2 Antibody (001)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DCUN1D2
Alternate Names
C13orf17, chromosome 13 open reading frame 17, DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae), DCN1-like protein 2, DCUN1 domain-containing protein 2, DCUN1L2, Defective in cullin neddylation protein 1-like protein 2, FLJ10704, FLJ20092
Gene Symbol
DCUN1D2
Additional DCUN1D2 Products
Product Documents for DCUN1D2 Antibody (001)
Product Specific Notices for DCUN1D2 Antibody (001)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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