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DCUN1D2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97034

Novus Biologicals, part of Bio-Techne
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NBP2-97034-100ul
NBP2-97034-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human DCUN1D2 (Accession#: NP_001014305.1; Met1-Phe259)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DCUN1D2 Antibody

Western Blot: DCUN1D2 Antibody [NBP2-97034]

Western Blot: DCUN1D2 Antibody [NBP2-97034]

Western Blot: DCUN1D2 Antibody [NBP2-97034] - Anti-DCUN1D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HCT116 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: DCUN1D2 Antibody [NBP2-97034]

Immunoprecipitation: DCUN1D2 Antibody [NBP2-97034]

Immunoprecipitation: DCUN1D2 Antibody [NBP2-97034] - DCUN1D2 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 2 ul anti-DCUN1D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DCUN1D2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa

Applications for DCUN1D2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCUN1D2

DCUN1D2 may contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-typecomplexes activity

Alternate Names

C13orf17, chromosome 13 open reading frame 17, DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae), DCN1-like protein 2, DCUN1 domain-containing protein 2, DCUN1L2, Defective in cullin neddylation protein 1-like protein 2, FLJ10704, FLJ20092

Gene Symbol

DCUN1D2

Additional DCUN1D2 Products

Product Documents for DCUN1D2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DCUN1D2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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