DDB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27207

Novus Biologicals, part of Bio-Techne
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NBP2-27207
NBP2-27207SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline, Primate

Predicted:

Chimpanzee (100%), Chinese Hamster (94%), Guinea Pig (94%), Orangutan (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all DDB1 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 385-435 from human DDB1 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Crab-eating Macaque, and 89% in Zebrafish, Cichlid fish, 83% in Chicken and Xenopus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDB1 Antibody

Western Blot: DDB1 Antibody [NBP2-27207]

Western Blot: DDB1 Antibody [NBP2-27207]

Western Blot: DDB1 Antibody [NBP2-27207] - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: DDB1 Antibody [NBP2-27207]

Flow Cytometry: DDB1 Antibody [NBP2-27207]

Flow Cytometry: DDB1 Antibody [NBP2-27207] - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.

Applications for DDB1 Antibody

Application
Recommended Usage

Flow Cytometry

0.2ug/10^6cells

Western Blot

2-4ug/ml~

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDB1

DNA Damage Binding protein 1 (DDB1) binds with CUL4 to form an ubiquitin E3 ligase that targets protein substrates for ubiquitin-dependent proteolysis (1, 2). Loss of DDB1 impedes the proteolysis of a grouping of CUL4 substrates at least in response to UV (3, 4) and CUL4 ubiquitinylation (5). DDB1 is highly conserved from yeast to mammals (3, 6). DDB1 specific antibody probes might be useful in identifying CUL4-DDB1 substrates in epigenetics applications and ubiquitin modification dependent protein function.

Long Name

Damage-specific DNA Binding Protein 1

Alternate Names

DDB p127 Subunit, DDBA, UV-DDB 1, XPCE

Gene Symbol

DDB1

UniProt

Q16531

Additional DDB1 Products

Product Documents for DDB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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