DDT Antibody (1G1)
Novus Biologicals, part of Bio-Techne | Catalog # H00001652-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1G1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DDT (AAH05971, 1 a.a. ~ 118 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL
Specificity
DDT - D-dopachrome tautomerase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for DDT Antibody (1G1)
![Western Blot: DDT Antibody (1G1) [H00001652-M01]](https://resources.bio-techne.com/images/products/DDT-Antibody-1G1-Western-Blot-H00001652-M01-img0003.jpg "Western Blot: DDT Antibody (1G1) [H00001652-M01]")
Western Blot: DDT Antibody (1G1) [H00001652-M01]
Western Blot: DDT Antibody (1G1) [H00001652-M01] - Analysis of DDT expression in transfected 293T cell line by DDT monoclonal antibody (M01), clone 1G1.Lane 1: DDT transfected lysate(12.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: DDT Antibody (1G1) [H00001652-M01] - Detection limit for recombinant GST tagged DDT is approximately 3ng/ml as a capture antibody.
Applications for DDT Antibody (1G1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DDT
Long Name
D-Dopachrome Tautomerase
Alternate Names
D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II
Entrez Gene IDs
1652 (Human)
Gene Symbol
DDT
OMIM
602750 (Human)
UniProt
Additional DDT Products
Product Documents for DDT Antibody (1G1)
Product Specific Notices for DDT Antibody (1G1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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