DDT Antibody (OTI1H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45677

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H3

Concentration

1 mg/ml

View all DDT Antibodies »

Product Summary for DDT Antibody (OTI1H3)

Immunogen

Full length human recombinant protein of human DDT(NP_001346) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDT Antibody (OTI1H3)

Western Blot: DDT Antibody (OTI1H3) [NBP2-45677]

Western Blot: DDT Antibody (OTI1H3) [NBP2-45677]

Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: DDT Antibody (OTI1H3) [NBP2-45677]

Immunohistochemistry: DDT Antibody (OTI1H3) [NBP2-45677]

Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DDT Antibody (OTI1H3) [NBP2-45677]

Western Blot: DDT Antibody (OTI1H3) [NBP2-45677]

Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.
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Applications for DDT Antibody (OTI1H3)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200 - 8000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDT

D-dopachrome decarboxylase, also known as D-dopachrome tautomerase (DDT), phenylpyruvate tautomerase II and DDCT, is a member of the migration inhibitory factor (MIF) family. DDT, via tautomerization and decarboxylation, converts D-dopachrome into 5,6-dihydroxyindole (DHI). DDT may also play a role in melanin biosynthesis.

Long Name

D-Dopachrome Tautomerase

Alternate Names

D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II

Gene Symbol

DDT

Additional DDT Products

Product Documents for DDT Antibody (OTI1H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDT Antibody (OTI1H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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