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DDX21 Antibody [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1718JF646

Novus Biologicals, part of Bio-Techne
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NB100-1718JF646

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Canine (100%), Equine (100%), Porcine (100%), Primate (100%), Rat (100%), Rhesus Macaque (100%), Squirrel (100%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Janelia Fluor 646

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, African elephant, Northern white-cheeked gibbon, and Thirteen-lined ground squirrel. Use in Zebrafish reported in scientific literature (PMID:32231306).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for DDX21 Antibody [Janelia Fluor® 646]

Application
Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DDX21

DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21, DKFZp686F21172, EC 3.6.1, EC 3.6.4.13, Gu protein, GUA, gu-alpha, GURDB, nucleolar RNA helicase 2, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu, RH-II/GU, RH-II/GuA, RNA helicase II/Gu alpha

Gene Symbol

DDX21

Additional DDX21 Products

Product Documents for DDX21 Antibody [Janelia Fluor® 646]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDX21 Antibody [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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