Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5B1

Concentration

1 mg/ml

View all DEC2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-297 of human BHLHE41(NP_110389) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DEC2/SHARP1 Antibody (OTI5B1)

Western Blot: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Western Blot: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Western Blot: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEC2.
Immunohistochemistry: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Immunohistochemistry: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Human lymph node tissue.
Immunohistochemistry: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Immunohistochemistry: DEC2/SHARP1 Antibody (OTI5B1) [NBP2-46574]

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Carcinoma of Human lung tissue.
View 4 more images

Applications for DEC2/SHARP1 Antibody (OTI5B1)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Application Notes
For IHC-P we recommend heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DEC2

Human DEC1 is a 412 amino acid, basic helix-loop-helix (bHLH) containing protein that is involved in the control of proliferation and/or differentiation of several cell types including nerve cells, fibroblasts and chondrocytes. The bHLH region of DEC1 is structurally similar to the bHLH regions of the mammalian HES family, Drosophila hairy, and Enhancer of split m7. DEC1 is a novel direct target for cAMP in a wide range of cells, and is involved in the control of gene expression in cAMP-activated cells. DEC2, also known as SHARP1, is highly expressed in skeletal muscle and brain. The gene encoding human DEC2 maps to chromosome 12p11.23-p12.1. DEC1 and DEC2 play a role in regulating the mammalian molecular clock by suppressing the transcription of specific clock genes. Both DEC1 and DEC2 are detected in the suprachiasmimc nucleus in a circadian fashion. Brief light impulses induce the expression of DEC1 in a phase-dependent manner.

Long Name

Differentially expressed in chondrocytes protein 2

Alternate Names

BHLHB3, BHLHE41, SHARP-1

Gene Symbol

BHLHE41

Additional DEC2 Products

Product Documents for DEC2/SHARP1 Antibody (OTI5B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DEC2/SHARP1 Antibody (OTI5B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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