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Decorin Antibody (DCN/6289)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20641

Novus Biologicals, part of Bio-Techne
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NBP3-20641-100ug
NBP3-20641-20ug

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine, Canine, Chicken, Elk, Equine, Rabbit

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # DCN/6289

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length Decorin protein

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Positive Controls: Prostate or skin tissue.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.

Scientific Data Images for Decorin Antibody (DCN/6289)

Decorin Antibody (DCN/6289)

Decorin Antibody (DCN/6289)

Formalin-fixed, paraffin-embedded human prostate stained with Decorin antibody (DCN/6289). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Decorin Antibody (DCN/6289)

Decorin Antibody (DCN/6289)

Formalin-fixed, paraffin-embedded human placenta stained with Decorin antibody (DCN/6289). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Decorin Antibody (DCN/6289)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Decorin

Decorin is encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Gene Symbol

DCN

Additional Decorin Products

Product Documents for Decorin Antibody (DCN/6289)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Decorin Antibody (DCN/6289)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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