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DEF6 Antibody (1F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00050619-M01

Novus Biologicals, part of Bio-Techne
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H00050619-M01

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DEF6 (AAH17504, 1 a.a. ~ 336 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLYTVLHIPHDPVALEEHFRDDDDGPVSSQGYMPYLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRADSNGNSMLSNQDAFRLWCLFNFLSEDKYPLIMVPDEVEYLLKKVLSSMSLEVSLGELEELLAQEAQVAQTTGGLSVWQFLELFNSGRCLRGVGRDTLSMAIHEVYQELIQDVLKQGYLWKRGHLRRNWAERWFQLQPSCLCYFGSEECKEKRGIIPLDAHCCVEVLPDRDGKRCMFCVKTATRTYEMSASDTRQRQEWTAAIQMAIRLQAEGKTSLHKDLKQKRREQREQR

Reactivity Notes

Human. Other species not tested.

Specificity

DEF6 - differentially expressed in FDCP 6 homolog (mouse)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DEF6 Antibody (1F2)

Immunohistochemistry-Paraffin: DEF6 Antibody (1F2) [H00050619-M01]

Immunohistochemistry-Paraffin: DEF6 Antibody (1F2) [H00050619-M01]

Immunohistochemistry-Paraffin: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of monoclonal antibody to DEF6 on formalin-fixed paraffin-embedded human lymph node Antibody concentration 3 ug/ml.
Sandwich ELISA: DEF6 Antibody (1F2) [H00050619-M01] - Detection limit for recombinant GST tagged DEF6 is approximately 1ng/ml as a capture antibody.
Immunoprecipitation: DEF6 Antibody (1F2) [H00050619-M01]

Immunoprecipitation: DEF6 Antibody (1F2) [H00050619-M01]

Immunoprecipitation: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of DEF6 transfected lysate using anti-DEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DEF6 monoclonal antibody.

Applications for DEF6 Antibody (1F2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM]

Alternate Names

DEF-6, differentially expressed in FDCP 6 homolog, differentially expressed in FDCP 6 homolog (mouse), IBPdifferentially expressed in FDCP (mouse homolog) 6, IRF4-binding protein, SLAT, SWAP70L, SWAP-70-like adaptor protein of T cells

Entrez Gene IDs

50619 (Human)

Gene Symbol

DEF6

Additional DEF6 Products

Product Documents for DEF6 Antibody (1F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DEF6 Antibody (1F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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