Skip to main content

DEF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-837

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB300-837

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence ALRKELLKSIWYA-C corresponding to N-Terminus according to NP_071330.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DEF6 Antibody

Western Blot: DEF6 Antibody [NB300-837]

Western Blot: DEF6 Antibody [NB300-837]

Western Blot: DEF6 Antibody [NB300-837] - Analysis of DEF6 on Human Thymus lysate using NB300-837 at 1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DEF6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human thymus lysates (calculated MW of 73.9 kDa band according to NP_071330.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Alternate Names

DEF-6, differentially expressed in FDCP 6 homolog, differentially expressed in FDCP 6 homolog (mouse), IBPdifferentially expressed in FDCP (mouse homolog) 6, IRF4-binding protein, SLAT, SWAP70L, SWAP-70-like adaptor protein of T cells

Entrez Gene IDs

50619 (Human); 23853 (Mouse)

Gene Symbol

DEF6

Additional DEF6 Products

Product Documents for DEF6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DEF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...