DEF8 Antibody (OTI4B6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00574
![Flow Cytometry: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Flow-Cytometry-NBP2-00574-img0002.jpg "Flow Cytometry: DEF8 Antibody (OTI4B6) [NBP2-00574]")
![Western Blot: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Western-Blot-NBP2-00574-img0003.jpg "Western Blot: DEF8 Antibody (OTI4B6) [NBP2-00574]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI4B6
Concentration
0.58 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DEF8 Antibody (OTI4B6)
Western Blot: DEF8 Antibody (OTI4B6) [NBP2-00574]
Western Blot: DEF8 Antibody (4B6) [NBP2-00574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.
Immunocytochemistry/Immunofluorescence: DEF8 Antibody (4B6) [NBP2-00574] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.
![Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0008.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]")
Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]
Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0004.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]")
![Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0005.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]")
![Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0006.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]")
![Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Immunohistochemistry-Paraffin-NBP2-00574-img0007.jpg "Immunohistochemistry-Paraffin: DEF8 Antibody (OTI4B6) [NBP2-00574]")
![Flow Cytometry: DEF8 Antibody (OTI4B6) [NBP2-00574]](https://resources.bio-techne.com/images/products/DEF8-Antibody-4B6-Flow-Cytometry-NBP2-00574-img0001.jpg "Flow Cytometry: DEF8 Antibody (OTI4B6) [NBP2-00574]")
Applications for DEF8 Antibody (OTI4B6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.58 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DEF8
Alternate Names
DEF-8, differentially expressed in FDCP 8 homolog, differentially expressed in FDCP 8 homolog (mouse), FLJ20186, MGC104349
Entrez Gene IDs
54849 (Human)
Gene Symbol
DEF8
Additional DEF8 Products
Product Documents for DEF8 Antibody (OTI4B6)
Product Specific Notices for DEF8 Antibody (OTI4B6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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