Dendritic Cell Marker Antibody (33D1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43284
![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.bio-techne.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0005.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 33D1
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was Dendritic Cell Marker.
Marker
Dendritic Cell Marker
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Scientific Data Images for Dendritic Cell Marker Antibody (33D1) - BSA Free
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the PE conjugate of NBP1-43284. Staining of BALB/c splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.125 ug of Rat IgG2b kappa Isotype Control PE (left) or 0.125 ug of Anti-Mouse Dendritic Cell Marker (33D1) PE (right).![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.bio-techne.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0001.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse Dendritic Cell Marker (33D1) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable c![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.bio-techne.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0004.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the Biotin conjugate of NBP1-43284. Staining of C57Bl/6 splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.25 ug of Rat IgG2b Isotype Control biotin (left) or 0.25 ug of bnti-Mouse Dendritic Cell Marker (33D1) Biotin (right) followed by Streptavidin PE.Applications for Dendritic Cell Marker Antibody (33D1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Dendritic Cell Marker
Alternate Names
DCs, Dendritic cell
Additional Dendritic Cell Marker Products
Product Documents for Dendritic Cell Marker Antibody (33D1) - BSA Free
Product Specific Notices for Dendritic Cell Marker Antibody (33D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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