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Deoxyribonuclease I like 1 Antibody (4E8)

Novus Biologicals, part of Bio-Techne | Catalog # H00001774-M02

Novus Biologicals, part of Bio-Techne
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H00001774-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DNASE1L1 (AAH01561, 18 a.a. ~ 302 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQLCPAA

Specificity

DNASE1L1 - deoxyribonuclease I-like 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Deoxyribonuclease I like 1 Antibody (4E8)

Western Blot: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02]

Western Blot: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02]

Western Blot: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02] - Analysis of DNASE1L1 expression in transfected 293T cell line by DNASE1L1 monoclonal antibody (M02), clone 4E8.Lane 1: DNASE1L1 transfected lysate(33.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02] - Detection limit for recombinant GST tagged DNASE1L1 is approximately 0.3ng/ml as a capture antibody.

Applications for Deoxyribonuclease I like 1 Antibody (4E8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Deoxyribonuclease I like 1

This gene encodes a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

Alternate Names

deoxyribonuclease I-like 1muscle-specific, DNase I-like 1, DNase X, DNASEX, DNL1LEC 3.1.21.-, EC 3.1.21, EC 3.1.21.1, Muscle-specific DNase I-like

Entrez Gene IDs

1774 (Human)

Gene Symbol

DNASE1L1

OMIM

300081 (Human)

Additional Deoxyribonuclease I like 1 Products

Product Documents for Deoxyribonuclease I like 1 Antibody (4E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Deoxyribonuclease I like 1 Antibody (4E8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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