DEPTOR/DEPDC6 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49674F

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-49674AF350

Alexa Fluor 405

NBP1-49674AF405

Alexa Fluor 488

NBP1-49674AF488

Alexa Fluor 532

NBP1-49674AF532

Alexa Fluor 594

NBP1-49674AF594

Alexa Fluor 647

NBP1-49674AF647

Alexa Fluor 700

NBP1-49674AF700

Alexa Fluor 750

NBP1-49674AF750

Allophycocyanin

NBP1-49674APC

Biotin

NBP1-49674B

CoraFluor 1

NBP1-49674CL1

DyLight 350

NBP1-49674UV

DyLight 405

NBP1-49674V

DyLight 488

NBP1-49674G

DyLight 550

NBP1-49674R

DyLight 594

NBP1-49674DL594

DyLight 650

NBP1-49674C

DyLight 680

NBP1-49674FR

DyLight 755

NBP1-49674IR

HRP

NBP1-49674H

Janelia Fluor 525

NBP1-49674JF525

Janelia Fluor 549

NBP1-49674JF549

Janelia Fluor 585

NBP1-49674JF585

Janelia Fluor 635

NBP1-49674JF635

Janelia Fluor 646

NBP1-49674JF646

Janelia Fluor 669

NBP1-49674JF669

mFluor Violet 450 SE

NBP1-49674MFV450

mFluor Violet 500 SE

NBP1-49674MFV500

mFluor Violet 610 SE

NBP1-49674MFV610

PE

NBP1-49674PE

PerCP

NBP1-49674PCP

Forumulation

Catalog #

BSA Free

NBP1-49674

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DEPTOR/DEPDC6 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human DEPTOR protein (within residues 10-200).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31176743).

Localization

Intracellular.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for DEPTOR/DEPDC6 Antibody [FITC]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DEPTOR/DEPDC6

DEPTOR (DEP domain-containing mTOR-interacting protein), also called DEP domain-containing protein 6 (DEPDC6), is a part of mTORC1 (MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR) and mTORC2 (MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR) complexes. DEPTOR functions as a negative regulator of the mTORC1/mTORC2 signaling pathways, leading to inhibition of activity of both kinase complexes. DEPTOR phosphorylation can weaken the interaction with MTOR within mTORC1 and mTORC2 complexes. DEPTOR has been shown to act as a tumor suppressor via mTORC1/mTORC2 blocking to inhibit protein synthesis as well as AKT's proliferation and survival related signaling. However, in certain conditions, DEPTOR behaves as an oncogene by relieving the feedback inhibition from S6K1 to PI3K, thus causing AKT activation. The stability of DEPTOR is controlled by SCFbeta-TrCP E3 ubiquitin ligase, and S6K1 and RSK1 are kinases that phosphorylate DEPTOR for facilitating its binding to betaTrCP for subsequent degradation. DEPTOR is down-regulated in a number of malignancies, however, is overexpressed in a subset of multiple myelomas harbouring cyclin D1/D3 or c-MAF/MAFB translocations.

Long Name

DEP Domain Containing mTOR-interacting Protein

Alternate Names

DEP.6, DEPDC6

Gene Symbol

DEPTOR

Additional DEPTOR/DEPDC6 Products

Product Documents for DEPTOR/DEPDC6 Antibody [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for DEPTOR/DEPDC6 Antibody [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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