DEPTOR/DEPDC6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85257

Novus Biologicals, part of Bio-Techne
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NBP1-85257
NBP1-85257-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DEPTOR/DEPDC6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DEPTOR/DEPDC6 Antibody

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Analysis in control (vector only transfected HEK293T lysate) and DEPTOR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Immunocytochemistry/ Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Immunocytochemistry/Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, mitochondria & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257]

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
View 2 more images

Applications for DEPTOR/DEPDC6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEPTOR/DEPDC6

DEPTOR is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of bothcomplexes

Long Name

DEP Domain Containing mTOR-interacting Protein

Alternate Names

DEP.6, DEPDC6

Entrez Gene IDs

64798 (Human)

Gene Symbol

DEPTOR

UniProt

Q8TB45 (Human)

Additional DEPTOR/DEPDC6 Products

Product Documents for DEPTOR/DEPDC6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DEPTOR/DEPDC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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