Desmin Antibody (DES/1711) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54503
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) - Azide and BSA Free [NBP2-54503]](https://resources.bio-techne.com/images/products/Desmin-Antibody-DES-1711-Immunohistochemistry-Paraffin-NBP2-54503-img0001.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) - Azide and BSA Free [NBP2-54503]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Hamster
Cited:
Mouse, Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DES/1711
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human desmin protein (Uniprot: P17661)
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34276989)
Localization
Cytoplasmic
Specificity
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Marker
Muscle Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53383).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Desmin Antibody (DES/1711) - Azide and BSA Free
Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) - Azide and BSA Free [NBP2-54503]
Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) [NBP2-54503] - Formalin-fixed, paraffin-embedded human Uterus stained with Desmin Monoclonal Antibody (DES/1711)Applications for Desmin Antibody (DES/1711) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34276989).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Desmin
Alternate Names
CMD1I, DES
Gene Symbol
DES
Additional Desmin Products
Product Documents for Desmin Antibody (DES/1711) - Azide and BSA Free
Product Specific Notices for Desmin Antibody (DES/1711) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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