Desmin Antibody (OTI4G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03539

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4G1

Concentration

0.63 mg/ml

View all Desmin Antibodies »

Product Summary for Desmin Antibody (OTI4G1)

Immunogen

Human fragment corresponding to amino acids 104-391 of human DES (NP_001918) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmin Antibody (OTI4G1)

Western Blot: Desmin Antibody (OTI4G1) [NBP2-03539]

Western Blot: Desmin Antibody (OTI4G1) [NBP2-03539]

Western Blot: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Desmin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Desmin.
Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] Staining of paraffin-embedded Human prostate tissue using anti-Desmin mouse monoclonal antibody.
Flow Cytometry: Desmin Antibody (OTI4G1) [NBP2-03539]

Flow Cytometry: Desmin Antibody (OTI4G1) [NBP2-03539]

Flow Cytometry: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Desmin antibody, and then analyzed by flow cytometry.
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Applications for Desmin Antibody (OTI4G1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.63 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Desmin

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq].

Alternate Names

CMD1I, DES

Entrez Gene IDs

1674 (Human)

Gene Symbol

DES

Additional Desmin Products

Product Documents for Desmin Antibody (OTI4G1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Desmin Antibody (OTI4G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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