Desmin Antibody (OTI4G1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03539
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4G1
Concentration
0.63 mg/ml
Product Specifications
Immunogen
Human fragment corresponding to amino acids 104-391 of human DES (NP_001918) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Desmin Antibody (OTI4G1)
Western Blot: Desmin Antibody (OTI4G1) [NBP2-03539]
Western Blot: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Desmin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Desmin.
Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] Staining of paraffin-embedded Human prostate tissue using anti-Desmin mouse monoclonal antibody.
Flow Cytometry: Desmin Antibody (OTI4G1) [NBP2-03539]
Flow Cytometry: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Desmin antibody, and then analyzed by flow cytometry.Applications for Desmin Antibody (OTI4G1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:200-500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.63 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Desmin
Additional Desmin Products
Product Documents for Desmin Antibody (OTI4G1)
Product Specific Notices for Desmin Antibody (OTI4G1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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