Desmin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06984
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RDGEVVSEATQQQHE corresponding to C-Terminus according to NP_001918.3.
Epitope
C Terminus
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 25575786).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Desmin Antibody
Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] -
Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: Desmin Antibody [NBP1-06984] -
Western Blot: Desmin Antibody [NBP1-06984] - (0.01ug/ml) staining of Human Skeletal Muscle (A) and (0.3ug/ml) Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: Desmin Antibody [NBP1-06984] -
Western Blot: Desmin Antibody [NBP1-06984] - (0.1ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Desmin Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
4-6 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.01-0.3 ug/ml
Application Notes
Western blot: Approx. 55-60kDa band observed in Human and Mouse Skeletal Muscle lysates and in Human Heart lysates (calculated MW of 53.5kDa according to Human NP_001918.3, and Mouse NP_034173.1). Preliminary testing showed a band at approx 60kDa in Rat and Pig Skeletal muscle lysate. Primary incubation 1 hour at room temperature. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 25575786).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Desmin
Alternate Names
CMD1I, DES
Gene Symbol
DES
UniProt
Additional Desmin Products
Product Documents for Desmin Antibody
Product Specific Notices for Desmin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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