Desmin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85549

Novus Biologicals, part of Bio-Techne
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NBP1-85549
NBP1-85549-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Desmin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Desmin Antibody

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-85549]

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-85549]

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-85549] - Staining in human heart muscle and liver tissues . Corresponding DES RNA-seq data are presented for the same tissues.
Western Blot: Desmin Antibody [NBP1-85549]

Western Blot: Desmin Antibody [NBP1-85549]

Western Blot: Desmin Antibody [NBP1-85549] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: Desmin Antibody [NBP1-85549]

Immunocytochemistry/ Immunofluorescence: Desmin Antibody [NBP1-85549]

Immunocytochemistry/Immunofluorescence: Desmin Antibody [NBP1-85549] - Staining of human cell line RH-30 shows localization to intermediate filaments. Antibody staining is shown in green.
View 4 more images

Applications for Desmin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmin

Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle.DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (Debus E et al.).

Alternate Names

CMD1I, DES

Gene Symbol

DES

Additional Desmin Products

Product Documents for Desmin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Desmin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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