Desmocollin-1 Antibody (JE66-57)

Western Blot: Desmocollin-1 Antibody (JE66-57) [NBP3-32264]

Western Blot: Desmocollin-1 Antibody (JE66-57) [NBP3-32264] - Western blot analysis of Desmocollin-1 on different lysates with Rabbit anti-Desmocollin-1 antibody (NBP3-32264) at 1/1,000 dilution.

Lane 1: C2C12 cell lysate
Lane 2: L6 cell lysate
Lane 3: RH-35 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 100 kDa
Observed band size: 100/60kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32264) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.